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Exploring the Effect of Curcumin on E. coli
Growth as a Tool to Battle Diarrheal
Disease in Developing Countries
anakumar
How does curcumin, organically found in
turmeric, affect E. coli growth as the
environmental determinant of E.
coli-related-diarrheal-diseases becomes a
growing health issue in developing countries?
E. coli bacterial colony growth will decrease in
turmeric -infused agar when compared to the
control agar culture.
O
• Escherichia coli, more often referred to as E. coli is a gram -negative anaerobic bacteria that
is commonly found in the lower intestine of healthy humans and animals.
• While most types of E. coli are harmless, a few strains such as E. coli O157:H7 can cause
severe symptoms of bloody diarrhea, vomiting, and potential kidney failure.
• There are six strains of E. coli that can cause diarrhea.
• In countries such as Africa where treated water is inaccessible, E. coli-caused death is an
issue. For example, in 2017, 606,024 deaths were caused by diarrheal diseases in
Sub -Saharan Africa.
• These diarrheal diseases are majorly correlated with the presence of E. coli.
• Curcuma, more commonly known as turmeric is a biologically active polyphenolic
compound in turmeric, and plays a significant role in the antioxidative property of turmeric.
• Curcumin has antioxidant, anti-inflammatory, and antimicrobial properties.
•
99% ethanol
•
Distilled water
•
Agar Agar Type 1
•
Mueller Hinton agar
•
Turmeric root
•
2 Petri dishes
•
E. coli culture
"° •
Inoculation tubes
•
Bunsen burner
•
Incubator
•
Autoclave
•
Mortar and pestle
•
Micropipette
• Sanitize all working surfaces with 99% ethanol
• Medium preparation:
0 0.65g of nutrient broth
0 50 mL of distilled water
• Sterilization to mix the medium
0 Autoclave medium at 121 degrees C for 20 mins
• Curcumin extract
o Grind raw turmeric with mortar and pestle
o Add 20 mL ethanol to the 2g raw turmeric
mixture
o Filter the mixture to extract the curcumin
• Media prep
0 Mix 1 g Agar Agar Type 1 and 3.8g Mueller
Hinton agar with 100 mL distilled water
• Sterilization of media
0 Autoclave media at 121 degrees C for 20 mins
• Pour 20 mL of the media into 2 sterile Petri plates
• After solidification of the media, swab 20-25µl
suspension of bacterial inoculums uniformly.
• Make wells on the agar medium using sterile
forceps.
• Pour turmeric extract into the wells according to the
2%, 5%, 10%, and 20% concentrations using a
pipette.
• Pour 10-40 µl of the turmeric extract into the wells
and Chloramphenicol antibiotic was used as
standard.
• Incubate Petri plates at 37-C for 24 hours.
• Measure the zone of inhibition from the edge of the
well to the zone in mm.
Zones of Inhibition According to Concentration of Turmeric Extract
Turmeric 2 pL 5 pL 10 pL 20 pL
Plate 1 Extract in
Wells (IuL)
Zone of 2mm 3mm 5mm 8mm
Inhibition
Turmeric 2 pL 5 pL 10 pL 20 pL
Plate 2 Extract in
Wells (pQ
Zone of 1 mm 1 mm 3 mm 5 mm
Inhibition
Result Analysis
0 The curcumin concentration
in turmeric averages at 3%.
• This low concentration of
curcumin is likely
responsible for the low
measurements for zones of
inhibition.
• If saturated curcumin was
used in the same
concentrations, there would
be a greater inhibition zone.
Zones of Inhibition According to Concentration of Turmeric Extract
® Plate 1 0 Plate 2
8
6
E
0
4
s
6 2
N
0
2 5 10 20
Amount of Turmeric Extract in Wells (NL)
• The data supports my hypothesis.
• However, the low concentration of
curcumin found in turmeric is responsible
for the lower -than -standard inhibition
zones.
• If higher concentrations of turmeric were
used with concentrated amounts of
curcumin, my hypothesis would be further
supported.
• As seen in the data, as the amount of
turmeric extract increases, the zones of
inhibitions increase, proving my claim that
higher concentrations of curcumin would
work better.
• Using high concentrations of curcumin can
be used to inhibit E. coli growth.
• The curcumin's antibacterial activity hasn't
been evaluated in clinical trials with an aim
of using it as a future antibiotic. Designing
medical drugs designed to treat E. coli
growth using curcumin extracted from
turmeric as an alternative to antibiotics can
be looked into.
• Additionally, exploring the inhibition of
turmeric in antibiotic -resistant E. coli
would further prove curcumin to be a
strong alternative.
Thank you
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