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Home My WebLink About05.b. Receive update on wastewater survellance for SARS-COV-2 Virus (the virus that causes COVID-19) Page 1 of 9 Item 5.b. ,orVIOIN SAN January 11, 2021 TO: REAL ESTATE, ENVIRONMENTAL AND PLANNING COMMITTEE FROM: BLAKE BROWN, SENIOR CHEMIST DAN FROST, SENIOR ENGINEER REVIEWED BY: MARY LOU ESPARZA, LABORATORY SUPERINTENDENT LORI SCHECTEL, ENVIRONMENTAL AND REGULATORY COMPLIANCE DIVISION MANAGER JEAN-MARC PETIT, DIRECTOR OF ENGINEERINGAND TECHNICAL SERVICES ROGER S. BAILEY GENERAL MANAGER SUBJECT: RECEIVE UPDATE ON WASTEWATER SURVEILLANCE FOR SARS-COV-2 VIRUS (THE VIRUS THAT CAUSES COVI D-19) Staff on Central San's COVI D-19 Technical Task Force will present an update on COVI D Wastewater Surveillance, including details on current studies Central San is participating in, sample collection, analysis, and preliminary results. Strategic Plan Tie-In GOAL ONE: Customer and Community Strategy 2—Maintain a positive reputation GOAL SIX: Innovation and Optimization Strategy 2—Improve and modernize operations through technology and efficiency measures GOAL SEVEN:Agility andAdaptability Strategy 1—Maintain a safe working environment for employees and the public during the COVID-19 pandemic ATTACHMENTS: 1. Presentation January 11, 2021 REEP Committee Meeting Agenda Packet- Page 13 of 49 Page 2 of 9 SARS-COV-2 SAMPLING AT CENTRAL SAN January 11, 2021 ---- Real Estate, Environmental and Planning Blake Brown, Senior Chemist Dan Frost, Senior Engineer e 'I CENTRAL SAN COVID-19 TECHNICAL TASK FORCE • Developed staff-led task force in April 2020 • Track and summarize the latest research of SARS- CoV-2 in wastewater: 1) wastewater-based epidemiology and surveillance 2) fate and transport of SARS-CoV-2 in wastewater • Explore and participate in research opportunities SARS-CoV-2 is the name of the virus that can cause COVID-19(the disease). 1 January 11, 2021 REEP Committee Meeting Agenda Packet- Page 14 of 49 Page 3 of 9 UNDERSTANDING SARS-COV-1 IN WASTEWATER No known transmissions of COVID-19 through wastewater exposure Experts believe SARS-CoV-2 virus does not survive long in wastewater Experts believe existing treatment processes are effective in removing the SARS-CoV-2 virus No known detections of SARS-CoV-2 Genetic fingerprint(RNA) in disinfected effluent RNA from SARS-CoV-2 can be detected and quantified in raw wastewater for trending purposes but not ready for predicting prevalence or confirming absence Wastewater surveillance is an efficient pooled sample of a community capturing asymptomatic individuals that are not captured by clinical tests, and trends could be detected several days ahead of clinical trends TASK FORCE INITIATED MULTIPLE EFFORTS IN THE LAST C MONTHS Routine Task Force National WasSurveillance withtewater Stanford Studies Department of Health and W*,Fp-- —49 (Fate&Transport) Human Services(HHS) Wastewater 7 - . - MolecularSurveillance with ' Task Contra Costa Health & I Archiving UC Berkeley Force Evaluated Sampling GT •... Confirm - : 2 January 11, 2021 REEP Committee Meeting Agenda Packet- Page 15 of 49 Page 4 of 9 STANFORD STUDIES S Stanford University • Stanford Tarpeh Study (long term) • Weekly samples at the Influent, Primary Sludge Peak Grab, Pre-UV Effluent, and Post-UV Effluent from June to September 2020 • Investigation of log-removal over the treatment process • Stanford Tarpeh Study (short term) • SARS-CoV-2 concentrations in hourly Influent diurnal flows at the start and end of Shelter-in-place • Results expected end of 2020 • Central San continues archiving samples for additional Stu GT MOLECULAR ESTIMATION OF POSITIVE COVID-19 CASES BASED ON SERVICE AREA POPULATION AND METADATA Sampling and Metadata • Collection on 9/4/2020 • Raw Influent 24-hour Composite (33.6 millions of gallons per day(MGD)) • 494,300 Population as of 1/1/2019(Source: California Department of Finance Demographic Research Unit) • Technology: Droplet Digital-PCR Data from Contra Costa Health Estimated COVI❑-19 Disease Prevalence' Services 1.07%prevalence rate' • On 9/4/2020, 7-day Rolling CASH ENmB1i0•Rirgr= 777$ t0 17711 alas Average %of new Tests R• We Rasa Emmatgn Range. 0.W 2.e9 s5 Positive 4.1 'EuinaxA C°YI°-19 Oieeaee WeWenee reluea aie dkuMeE ueYq Te medM pW1eME n Weetaww Data should not be used as a SARSCeV-z�eaeae,�a9ewanro�et�ar, °ese��.��e ee�b,er�ee °weaa inmprcwlN30i. direct comparison to Contrame.Tenn„�aM�.�„d,,,m,,, „�,aeMe a��aeedoaee,ae ate . Costa County Case Data `°' xy��neeengaeenreenowwe.reuee.ecwio-t9d.:cnmebe o-aee,eue NA...•��,�.t,.o,tn...�a,ram��.� ,..�� GT Molecular Report ' S 3 January 11, 2021 REEP Committee Meeting Agenda Packet- Page 16 of 49 Page 5 of 9 UNDERSTANDING WASTEWATER SERVICE AREA AND COUNTY HEALTH JURISDICTION • Contra Costa County Treatment Plant population: L 1,160,099 ' Central San Service Area r' • Central San Contra C County service area: JaAland c J 494,300 rranuscor, .. n•.,,, r' 5 Hospitals Central San serves —50% of County I7 ARCHIVED FROZEN SAMPLES • Influent samples collected weekly from 6/12/2020 - 9/14/2020 • Berkeley Pop-up lab analyzed 1 liter(L)from each sampling event • The second 1 L is still in archive awaiting another potential study • Primary Sludge Peak Grabs • Collected weekly from 6/12/2020- 9/14/2020 • Awaiting another potential study _ to compare to Stanford data 8 4 January 11, 2021 REEP Committee Meeting Agenda Packet- Page 17 of 49 Page 6 of 9 BERKELEY COVID-WEB • Objectives of the Berkeley Testing Program • Grant money is funding the effort(no cost to Central San) • Perform COVID-19 analysis of wastewater with 1 —2 day turn-around time • Communicate real-time data to local public health departments for trending • All data are available through the portal COVID-WEB • Other Contra Costa County Publicly Owned Treatment Works Participants • Delta Diablo • West County Wastewater District • Who will be using the data? • Contra Costa Health Services ' 9 BERKELEY AND CONTRA COSTA HEALTH SERVICES SURVEILLANCE SAMPLING How is Central San participating? • 3 times per week: 24-hour Flow weighted Composite Raw Influent • 1 time per week: 24-hour Time weighted Composite at Concord Main Metering Station (Targeted collection: 80% of Concord flows and 100% of Clayton flows) 67 a.. � io 5 January 11, 2021 REEP Committee Meeting Agenda Packet- Page 18 of 49 Page 7 of 9 METADATA COMPILATION COVID-19 Wastewater Epidemiologyfor the Bay Area Through U.C.Berkeley Sample Log and Metadata chart Treatment Facility:Central Contra Costa Sanitary District Co I I ected/Released by:Blake Brown Sample Motes: Collection Collection Sample Pre- Flow CBOD TSS Tube Label Sample Location Date Time treatment [MGD) (mg/L) (mg/L) AA83151 Raw Influent 24h Comp. 06/12/20 8:20 AM none 33.4 184 272 AA83373 Raw Influent 24h Comp. 06/19/20 8:14AM none 33 221 262 AA83581 Raw Influent 24h Comp. 06/26/20 8:16 AM none 32.9 213 296 AA83739 Raw Influent 24h Comp. 07/02/20 8:11 AM none 32 196 284 AA83975 Raw Influent 24h Comp. 07/10/20 8:54 AM none 34.6 243 224 AA84185 Raw Influent 24h Comp. 07/17/20 8:20 AM none 33.8 234 280 AA84377 Raw Influent 24h Comp. 07/24/20 8:38 AM none 34.1 271 AA84591 Raw Influent 24h Comp. 07/31/20 8:31 AM none 32.7 220 310 AA84793 Raw Influent 24h Comp. 08/07/20 8:32 AM none 33.2 187 276 AA85014 Raw Influent 24h Comp. 08/14/20 8:33 AM none 32.7 250 282 AA85206 Raw Influent 24h Comp. 08/21/20 8:35 AM none 32.1 220 272 AA85397 Raw Influent 24h Comp. 08/28/20 7:44 AM none 32.6 198 265 AA85628 Raw Influent 24h Comp. 09/04/20 9:04AM none 33.6 226 Mnre�m Ime) I11 PRELIMINARY BERKELEY RESULTS ARCHIVED SAMPLES FROM 6/12/2020 -9/4/2020 �• • Data are preliminary _�. • Interpretation is ongoing • Data are not - representative of all of CoCo County (only -50%) T-Day Rn111n9 AV.re•e•or N.C.by C.11-1un Day Geo a0n Source:Coronavirus.cchealth.org/overview 12 6 January 11, 2021 REEP Committee Meeting Agenda Packet- Page 19 of 49 Page 8 of 9 BERKELEY PRELIMINARY DATA 11/17/2020 - 12/21/2020 UP- tea. 3 • o o DA • _ quaff Qoscore o s• E • • de[ecte .f_3_TechReps ♦ O i • o 3 C o G o I o l's�♦'{c .,4 9c��A Pte'1.4 _'' ,yl. .y' ry� .y4 .,9p M1P4 y>4 ,�^• ,�' • N1 Graph shows Gene copies per milliliter(mL)of SARS-CoV-2 in wastewater • PMMoV Graph shows Gene copies per mL of PMMoV(pepper mild mottle virus) used as a fecal indicator virus tracer to normalize concentrations of SARS-CoV-2 to account for variation in the wastewater strength (i.e.,the amount by which fecal waste is diluted by other sources of wastewater) ' is NATIONAL HHS COVID-19 WASTEWATER SURVEILLANCE PROJECT • AquaVitas, LLC was awarded the contract through HHS on 11/13/20 • AquaVitas will enter results into the National Wastewater Surveillance System (NWSS)to HHS • Centers for Disease Control and Prevention (CDC)will pull data into NWSS DCIPHER portal for analysis • After review, CDC will communicate data to state health departments • Objective: to monitor 100 million people and test up to 30% of the country's wastewater(about 100 treatment plants over 42 states) • Phase 1 • January-February(8 weeks total) • Twice per week influent(Monday/wednesday) • Phase 2 Recruitment will start in January 2021 14 7 January 11, 2021 REEP Committee Meeting Agenda Packet- Page 20 of 49 Page 9 of 9 THANK YOU • Laboratory • Planning and Development • Environmental Compliance • Instrument Shop and Buildings and Grounds • Operations • Regulatory Compliance Group I15 8 January 11, 2021 REEP Committee Meeting Agenda Packet- Page 21 of 49